LH DETECT® for buffaloes

Rapid tests

Assay kits

Service provision

Cattle

Pig

Sheep

Goat

Dog

Others

Project

Head office:
Centre INRAe Val de Loire
37380 Nouzilly, France

Phone:
+33 (0) 2 47 42 79 35

Email:
contact@repropharm.com

repropharm.christian

LH DETECT® for buffaloes

^{LH DETECT^® for buffaloes}

An immuno-enzymatic (not radio-active) assay kit to accurately measure Luteinizing Hormone in various animal species.

LH DETECT® can be used in a laboratory for highly accurate measurements and detection of the pre-ovulatory LH surge, from a blood, serum or plasma sample. For sheep and goats, measurement can also be done on a milk sample.

Description

LH DETECT® is particularly useful for the following works :

Following the LH secretions during the male and female sexual cycle
Studying the micropulsatility of LH secretions
Determining the most appropriate time to proceed to an artificial insemination insofar as the time between LH surge and ovulation is known and constant for each specie
Any other study requiring an accurate LH measurement

LH detection can be done from blood, plasma or serum samples, and even in the milk of sheeps.

Choose your substrate: ABTS or TMB (recommanded for quantitative works); and your kind of plate: entire one or strip one (divisible in 16-well segments).

LH DETECT® is build under an exclusive and wordlwide INRA licence.

Principle

LH DETECT® is an ELISA sandwich type immunoenzymatic test. The enzymatic activity is revealed by a chromogenic substrate.

Reaction intensity is proportionnal to LH concentration in the test sample. They evaluation may be performed visually (qualitative) or measured with a spectrophotometer.

For qualitative assay, a positive control sample permits the reaction validation. For this use, we recommend using ABTS as substrate.

For quantitative assay, a scale of standards, produced with the LH of the concerning specie, is used. In this case, we recommend using TMB as substrate.

Procedure

Please find below the schematic procedure of LH DETECT®. It describes all the procedure, including sample and reagents preparation.

More detailed data are sent with any product order through our website. For any question regarding to the use of LH DETECT®, please ask to our technical team by using the 'Contact' email.

Download the schematic procedure

Applications

LH DETECT® can be used among buffaloes as a quantitative LH measurement as well as a qualitative detection. Some works were realised among italian buffaloes in order to define the protocols for synchronisation treatments and artificial insemination timing. The blood samplings rate is 1 every 4 hours, from the œstrus beginning to the LH surge detection, between 48 to 72 hours (3 days).