Head office:
Centre INRAe Val de Loire
37380 Nouzilly, France
Phone:
+33 (0) 2 47 42 79 35
Email:
contact@repropharm.com
| LH DETECT® for cervidae
An immuno-enzymatic (not radio-active) assay kit to accurately measure Luteinizing Hormone in various animal species.
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LH DETECT® is particularly useful for the following works :
LH detection can be done from blood, plasma or serum samples, and even in the milk of sheeps.
Choose your substrate: ABTS or TMB (recommanded for quantitative works); and your kind of plate: entire one or strip one (divisible in 16-well segments).
LH DETECT® is build under an exclusive and wordlwide INRA licence.
LH DETECT® is an ELISA sandwich type immunoenzymatic test. The enzymatic activity is revealed by a chromogenic substrate.
Reaction intensity is proportionnal to LH concentration in the test sample. They evaluation may be performed visually (qualitative) or measured with a spectrophotometer.
For qualitative assay, a positive control sample permits the reaction validation. For this use, we recommend using ABTS as substrate.
For quantitative assay, a scale of standards, produced with the LH of the concerning specie, is used. In this case, we recommend using TMB as substrate.
You can choose between two types of plate: entire one, or strip one divisible in 16-well segments. The strip one allows avoiding defrosting all the wells when only a few samples are going to be tested.
Please find below the schematic procedure of LH DETECT®. It describes all the procedure, including sample and reagents preparation.
More detailed data are sent with any product order through our website. For any question regarding to the use of LH DETECT®, please ask to our technical team by using the 'Contact' email.
Download the schematic procedure
LH DETECT® was used among Rusa doe from New Caledonia. The purpose was to optimize the treatments for the control of cycles and the artificial insemination protocols among this specie.
An other major application is to determine the best moment for artificial inseminations after superovulation treatments among embryos-producing females. This is particularly important for the safeguard of endangered cervidae races, for which the only way to make them durable is to treat females to induce superovulation, to inseminate them, and then to transplant the embryos to conductress females from an non endangered and compatible race.
Among this specie, the preovulatory LH surge duration is about 18 to 20 hours. The LH surge beginning happens at a changing time from the heats beginning. Blood samplings were realised every 2 hours during the heats period.
